How to remove oil/fat from casein other than oil-extraction method ?

By manipulating the pH of a water solution you can dissolve Casein in an aqueous solution. Once done separate the phases. The Casein can be recovered by further manipulation of the pH.





NOTE: Casein forms very thick solution with water so be prepared with a strong stirrerHow to remove oil/fat from casein other than oil-extraction method ?
1. Most samples of casein available in the market may not have any fat / oil. It has already been removed.


2.If you are preparing casein from milk and you want to remove oil /fat. Whatever method you may employ it will be extraction of fat/oil. Afterall you want to separate oil/fat from casein.


3. You have a choice of organic liquid solvents like ether, chloroform. dichloromethane, etc. Or even super critical CO2.


4. Even by manuplating pH. you need to remove oil /fat from the solution. I think it will still need a step of solvent extration.How to remove oil/fat from casein other than oil-extraction method ?
by fractional distillation of oil